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Phase Optical Microscope - Contrast
This microscopic technique increases the contrast of transparent specimens, living cells, microorganisms, and other specimens. The main advantage of the contrast phase technique is that living cells and tissues do not need to be lost, stained, painted or prepared in any way, and can be examined with their normal state. Dynamic recording and analysis of complex biological processes is very easy with the phase contrast microscope.
A phase contrast image usually contains a background with a natural color, surrounded by variable contrast created by the effect of the sample on the light. The two most commonly used effects in the contrast phase images are shadow patterns and light halo. This occurs when the focal length of the Mordaj-Infinity is not consistent with the sample and the background. Although these effects are common in the contrast phase images, they reduce the resolution of the detail. Specific object lenses, called apodizing phase contrast objects, are used to reduce this effect.
Phase Optical Microscope - Contrast | Polaritc Company

Figure 6) Contrast Picture of a Napkin Napkin
The contrast phase technique translates very little changes in the wave phase to discriminating domain variations. A bright field microscope can be converted into a contrast phase microscope by adding two pieces. These two pieces are:
Fuzzy page: By placing on the back of the object's lens, this page selectively changes the phase and range of the rays passing through the sample.
Condenser ring: A black flat plate has a transparent ring placed in the front of the focal plane.
Phase Optical Microscope - Contrast | INFORTEX COMPANY

Figure 7 Optical path microscope Contrast Phase