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Optical Fluorescence Microscope
Fluorescence microscope is a technique used by fluoroscopic fluorescence radiation created by fluorophors. Fluorophore is a kind of fluorescence pigment used to label proteins, tissues and cells to see them using a fluorescence microscope. The fluorophore absorbs the light at a particular wavelength (arousal), and then reflects this energy in a different optical wavelength (emission wavelength).
Fluorescence microscopes can have a simple system like epifluorescent microscopes, or have a complex structure like a multi-focal or multi-photon microscope. This simple and complex microscope follows the same principles. An excitation energy is used to irradiate a specimen. Then this energy is emitted by a different wavelength sample. The radiation emitted, although weak, can be measured. The excitation and emission wavelengths are not the same as the wavelengths, so the overall opacity can be increased with optical filters.
The following figure shows a real fluorescent image. The specimen is excited by a low-wavelength source (350-350 nm) and then emits a reflection wavelength that is longer than the astronomical wavelength. Images like this can not be seen without the use of advanced light filtering techniques. Fluorescent proteins in the samples produce outstanding colors.
Optical Fluorescence Microscope INFORTEX COMPANY

Figure 8) Fluorescence image of micro-spheres
Fluorescent microscopes are very similar to dark field microscopes and bright fields. With the difference in fluorescence microscopy, sophisticated filtering techniques are used to narrow the narrow band of light. The three key filters required for fluorescence microscopy include an excitation filter, two-color, and emission.
Aperture filter: This filter is placed in the exposure pathway to the sample. This filters all wavelengths of the light source, except the wavelengths that cause the fluorophore excitation or the underlying sample to be evaluated.
Double-colored filter: With a 45 degree angle between the evaporation filter and the exhaust filter. This filter reflects the rays of arousal toward the fluorophore and passes through the reflection beams to reach the sensor.
Exhaust Filter: This filter is placed in the sample path of the sample. This piece filters the entire reflection rays of the sample and allows it to pass only to the reflected beams.
Optical Fluorescence Microscope INFORTEX COMPANY

Figure 9: Configuration of optical filters used in fluorescence microscopy