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Optical dark microscope
The dark field exposure is a technique that removes scattered light from the sample in the image. So the image obtained by this microscopic technique, unlike the bright background, is a typical image on a dark background. The primary purpose of imaging by this technique is to increase the contrast of the colored specimens.
Common images of dark field microscopes include a bright example in a dark background. This method is suitable for capturing non-colored samples or images that require contrast enhancement. The advantage of this method is that living specimens do not need to stain and can remain nasty. Also, due to live cellular analysis, it is possible to obtain more qualitative results in this method.
Optical dark microscope INFORTEX COMPANY

Figure 2) Illuminating the dark background of the tissue paper
 

Figure 3) Optical path of the dark field microscope

Optical path A dark field microscope is usually applied vertically. This path consists of three key pieces:
Light source: This light opens to the compacting block by colliding with the blocking disc, and only one loop hits the sample.
Density Lens: The light passes through the circle that passes through the disk and builds it on the focal sample.
Object Lens: Displays scattered light from the specimen. It should be noted that the light passed through the specimen, if not scattered, is not collected by this lens.